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KMID : 0922220110110020055
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Journal of the Korean Musculoskeletal Transplantation Society
2011 Volume.11 No. 2 p.55 ~ p.60
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Difference of Platelet Rich Plasma Formation by Various Centrifugation Speed and Time in Rab
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Park Chang-Goo
Park Sang-Won
Lee Da-Reum
Jeong Jin-Young
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Abstract
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Purpose: The purpose of this study was to examine the platelet concentration and the recovery ratio of PRP by centrifugation time and gravitational force (g) in rabbit.
Materials and Methods: 6 New Zealand white rabbit weighted 2.5~3 kg were divided into three groups. 39 blood samples of 9 ml each were collected. Each experimental groups were divided into three groups by centrifugation time and gravitational force. A group was tested at step 1: 150 g for 20 min, step 2: 450 g for 10 min, B group was tested at step 1: 900 g for 5 min, step 2: 1500 g for 15 min and C group was tested at step 1: 2000 g for 3 min, step 2: 5000 g for 5min. Each groups were compared the platelet concentration and recovery ratio.
Results: After step 1 centrifugation, A group noted 254.2±36.7×103/μl and 65.7±14.3 % in concentration and recovery ratio and came out the lowest. B group noted 578.9±116.9×103/μl and 91.2±13.1% in concentration and recovery ratio and came out the highest. C group noted 247.1±37.4×103/μl and 72.4±6.5% in concentration and recovery ratio. The total platelet counts were 1047, 2316, 1811 respectively and B group was stastically significant(p<0.05). After step 2 centrifugation, platelet counts were 1014.9±168.5×103/μl, 1486.0±266.4×103/μl and 1067.5±259.6×103/μl and recovery ratios were 62.1±9.3%, 65.2±10.7% and 59.0±11.9%. B group shows highest value but no stastical significance(p>0.05).
Conclusion: During the formation of platelet rich plasma in rabbit, the step 1 centrifugation with 900 g for 5 minutes and it shows the highest platelet concentration. After the step 2 centrifugation, although each group shows no significant difference, the recovery ratio came out the highest at centrifugation with 1500 g for 15 minutes.
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KEYWORD
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Platelet rich plasma, Recovery ratio
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